Tryptophan-based fluorophores for studying protein conformational changes
نویسندگان
چکیده
منابع مشابه
A fluorescent probe designed for studying protein conformational change.
The usefulness of fluorescence in studying protein motions derives from its sensitivity, kinetic resolution, and compatibility with both live cells and physiological assays. Recent advances in microscopy and membrane protein purification have permitted the observation of fluorescence changes that accompany the functional transitions of complex eukaryotic membrane proteins. These techniques rely...
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E. coli FtsZ has no native tryptophan. We showed previously that the mutant FtsZ L68W gave a 2.5-fold increase in trp fluorescence when assembly was induced by GTP. L68 is probably buried in the protofilament interface upon assembly, causing the fluorescence increase. In the present study we introduced trp residues at several other locations and examined them for assembly-induced fluorescence c...
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Motions related to protein-protein binding events can be surveyed from the perspective of the Database of Macromolecular Movements. There are a number of alternative conceptual models that describe these events, particularly induced fit and pre-existing equilibrium. There is evidence for both alternatives from recent studies of conformational change. However, there is increasing support for the...
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PURPOSE To investigate whether any of the fluorophores in the human lens nuclei might be responsible for human brunescent cataract formation. METHODS Human lens nuclei (non-brunescent, from 13; brunescent, from 8) were obtained after extracapsular cataract extraction in nondiabetic patients. Protein-free extract, prepared by filtrating the water-soluble fraction of each nucleus through a cent...
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In a purified system containing poly(U) and ribosomal subunits from Escherichia coli, and purified transfer factors T and G, the active ribosomal complex passes through a cycle of contraction and expansion with the addition of each amino acid; aminoacyl-tRNA binding catalyzed by T produces the stable compact state, corresponding to the 70S conformation, whereas translocation with G expands the ...
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ژورنال
عنوان ژورنال: Bioorganic & Medicinal Chemistry
سال: 2014
ISSN: 0968-0896
DOI: 10.1016/j.bmc.2014.09.015